Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry
Identifieur interne : 000182 ( Main/Exploration ); précédent : 000181; suivant : 000183Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry
Auteurs : Jennifer Pasquier [États-Unis] ; Damien Rioult ; Nadine Abu-Kaoud ; Jessica Hoarau-Véchot ; Matthieu Marin ; Frank Le FollSource :
- International Journal of Molecular Sciences [ 1422-0067 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : P-Glycoprotein.
- chemical , metabolism : Chloride Channels, P-Glycoprotein.
- methods : Flow Cytometry.
- Cell Size, Humans, MCF-7 Cells.
Abstract
The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.
Url:
DOI: 10.3390/ijms160714318
PubMed: 26114386
PubMed Central: 4519844
Affiliations:
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E-Mail:<email>matthieu.marin@univ-lille1.fr</email></nlm:aff></affiliation></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name><affiliation><nlm:aff id="af5-ijms-06-14318">Laboratory of Ecotoxicology UPRES EA 3222, IFRMP 23, University of Le Havre, 76058 Le Havre, France; E-Mail:<email>frank.lefoll@univ-lehavre.fr</email></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26114386</idno><idno type="pmc">4519844</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519844</idno><idno type="RBID">PMC:4519844</idno><idno type="doi">10.3390/ijms160714318</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000008</idno><idno type="wicri:Area/Pmc/Curation">000008</idno><idno type="wicri:Area/Pmc/Checkpoint">000023</idno><idno type="wicri:source">PubMed</idno><idno type="wicri:Area/PubMed/Corpus">000084</idno><idno type="wicri:Area/PubMed/Curation">000084</idno><idno type="wicri:Area/PubMed/Checkpoint">000084</idno><idno type="wicri:Area/Ncbi/Merge">000483</idno><idno type="wicri:Area/Ncbi/Curation">000483</idno><idno type="wicri:Area/Ncbi/Checkpoint">000483</idno><idno type="wicri:Area/Main/Merge">000180</idno><idno type="wicri:Area/Main/Curation">000182</idno><idno type="wicri:Area/Main/Exploration">000182</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry</title><author><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name><affiliation><nlm:aff id="af1-ijms-06-14318">Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha 24144, Qatar; E-Mails:<email>naa2037@qatar-med.cornell.edu</email> (N.A.-K.);<email>jeh2036@qatar-med.cornell.edu</email> (J.H.-V.)</nlm:aff></affiliation><affiliation wicri:level="2"><nlm:aff id="af2-ijms-06-14318">Department of Genetic Medicine, Weill Cornell Medical College, New York, NY 10022, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Genetic Medicine, Weill Cornell Medical College, New York, NY 10022</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name><affiliation><nlm:aff id="af3-ijms-06-14318">UMR_I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et BIOsurveillance des milieux aquatiques, Université de Reims Champagne-Ardenne, BP-1039-Reims Cedex 2, 51687 Reims, France; E-Mail:<email>damien.rioult@univ-reims.fr</email></nlm:aff></affiliation></author><author><name sortKey="Abu Kaoud, Nadine" sort="Abu Kaoud, Nadine" uniqKey="Abu Kaoud N" first="Nadine" last="Abu-Kaoud">Nadine Abu-Kaoud</name><affiliation><nlm:aff id="af1-ijms-06-14318">Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha 24144, Qatar; E-Mails:<email>naa2037@qatar-med.cornell.edu</email> (N.A.-K.);<email>jeh2036@qatar-med.cornell.edu</email> (J.H.-V.)</nlm:aff></affiliation></author><author><name sortKey="Hoarau Vechot, Jessica" sort="Hoarau Vechot, Jessica" uniqKey="Hoarau Vechot J" first="Jessica" last="Hoarau-Véchot">Jessica Hoarau-Véchot</name><affiliation><nlm:aff id="af1-ijms-06-14318">Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha 24144, Qatar; E-Mails:<email>naa2037@qatar-med.cornell.edu</email> (N.A.-K.);<email>jeh2036@qatar-med.cornell.edu</email> (J.H.-V.)</nlm:aff></affiliation></author><author><name sortKey="Marin, Matthieu" sort="Marin, Matthieu" uniqKey="Marin M" first="Matthieu" last="Marin">Matthieu Marin</name><affiliation><nlm:aff id="af4-ijms-06-14318">UGSF, UMR CNRS 8576, équipe Régulation des Signaux de Division, Université de Lille, Sciences et Technologies, 59009 Villeneuve d’Ascq, France; E-Mail:<email>matthieu.marin@univ-lille1.fr</email></nlm:aff></affiliation></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name><affiliation><nlm:aff id="af5-ijms-06-14318">Laboratory of Ecotoxicology UPRES EA 3222, IFRMP 23, University of Le Havre, 76058 Le Havre, France; E-Mail:<email>frank.lefoll@univ-lehavre.fr</email></nlm:aff></affiliation></author></analytic><series><title level="j">International Journal of Molecular Sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Size</term><term>Chloride Channels (metabolism)</term><term>Flow Cytometry (methods)</term><term>Humans</term><term>MCF-7 Cells</term><term>P-Glycoprotein (genetics)</term><term>P-Glycoprotein (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>P-Glycoprotein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chloride Channels</term><term>P-Glycoprotein</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Flow Cytometry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Size</term><term>Humans</term><term>MCF-7 Cells</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Okada, Y" uniqKey="Okada Y">Y. Okada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Okada, Y" uniqKey="Okada Y">Y. Okada</name></author><author><name sortKey="Maeno, E" uniqKey="Maeno E">E. Maeno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Agre, P" uniqKey="Agre P">P. Agre</name></author><author><name sortKey="King, L S" uniqKey="King L">L.S. King</name></author><author><name sortKey="Yasui, M" uniqKey="Yasui M">M. Yasui</name></author><author><name sortKey="Guggino, W B" uniqKey="Guggino W">W.B. Guggino</name></author><author><name sortKey="Ottersen, O P" uniqKey="Ottersen O">O.P. Ottersen</name></author><author><name sortKey="Fujiyoshi, Y" uniqKey="Fujiyoshi Y">Y. Fujiyoshi</name></author><author><name sortKey="Engel, A" uniqKey="Engel A">A. Engel</name></author><author><name sortKey="Nielsen, S" uniqKey="Nielsen S">S. Nielsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sardini, A" uniqKey="Sardini A">A. Sardini</name></author><author><name sortKey="Amey, J S" uniqKey="Amey J">J.S. Amey</name></author><author><name sortKey="Weylandt, K H" uniqKey="Weylandt K">K.H. Weylandt</name></author><author><name sortKey="Nobles, M" uniqKey="Nobles M">M. Nobles</name></author><author><name sortKey="Valverde, M A" uniqKey="Valverde M">M.A. Valverde</name></author><author><name sortKey="Higgins, C F" uniqKey="Higgins C">C.F. Higgins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lang, F" uniqKey="Lang F">F. Lang</name></author><author><name sortKey="Busch, G L" uniqKey="Busch G">G.L. Busch</name></author><author><name sortKey="Volkl, H" uniqKey="Volkl H">H. Volkl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peak, M" uniqKey="Peak M">M. Peak</name></author><author><name sortKey="Al Habori, M" uniqKey="Al Habori M">M. al-Habori</name></author><author><name sortKey="Agius, L" uniqKey="Agius L">L. Agius</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kroemer, G" uniqKey="Kroemer G">G. Kroemer</name></author><author><name sortKey="Galluzzi, L" uniqKey="Galluzzi L">L. Galluzzi</name></author><author><name sortKey="Vandenabeele, P" uniqKey="Vandenabeele P">P. Vandenabeele</name></author><author><name sortKey="Abrams, J" uniqKey="Abrams J">J. Abrams</name></author><author><name sortKey="Alnemri, E S" uniqKey="Alnemri E">E.S. Alnemri</name></author><author><name sortKey="Baehrecke, E H" uniqKey="Baehrecke E">E.H. Baehrecke</name></author><author><name sortKey="Blagosklonny, M V" uniqKey="Blagosklonny M">M.V. Blagosklonny</name></author><author><name sortKey="El Deiry, W S" uniqKey="El Deiry W">W.S. El-Deiry</name></author><author><name sortKey="Golstein, P" uniqKey="Golstein P">P. Golstein</name></author><author><name sortKey="Green, D R" uniqKey="Green D">D.R. Green</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Okada, Y" uniqKey="Okada Y">Y. Okada</name></author><author><name sortKey="Maeno, E" uniqKey="Maeno E">E. Maeno</name></author><author><name sortKey="Shimizu, T" uniqKey="Shimizu T">T. Shimizu</name></author><author><name sortKey="Dezaki, K" uniqKey="Dezaki K">K. Dezaki</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Morishima, S" uniqKey="Morishima S">S. Morishima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosch, I" uniqKey="Bosch I">I. Bosch</name></author><author><name sortKey="Croop, J" uniqKey="Croop J">J. Croop</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Endicott, J A" uniqKey="Endicott J">J.A. Endicott</name></author><author><name sortKey="Ling, V" uniqKey="Ling V">V. Ling</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jentsch, T J" uniqKey="Jentsch T">T.J. Jentsch</name></author><author><name sortKey="Gunther, W" uniqKey="Gunther W">W. Gunther</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nilius, B" uniqKey="Nilius B">B. Nilius</name></author><author><name sortKey="Eggermont, J" uniqKey="Eggermont J">J. Eggermont</name></author><author><name sortKey="Voets, T" uniqKey="Voets T">T. Voets</name></author><author><name sortKey="Droogmans, G" uniqKey="Droogmans G">G. Droogmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valverde, M A" uniqKey="Valverde M">M.A. Valverde</name></author><author><name sortKey="Diaz, M" uniqKey="Diaz M">M. Diaz</name></author><author><name sortKey="Sepulveda, F V" uniqKey="Sepulveda F">F.V. Sepulveda</name></author><author><name sortKey="Gill, D R" uniqKey="Gill D">D.R. Gill</name></author><author><name sortKey="Hyde, S C" uniqKey="Hyde S">S.C. Hyde</name></author><author><name sortKey="Higgins, C F" uniqKey="Higgins C">C.F. Higgins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Horton, J K" uniqKey="Horton J">J.K. Horton</name></author><author><name sortKey="Vanoye, C G" uniqKey="Vanoye C">C.G. Vanoye</name></author><author><name sortKey="Reuss, L" uniqKey="Reuss L">L. Reuss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hardy, S P" uniqKey="Hardy S">S.P. Hardy</name></author><author><name sortKey="Goodfellow, H R" uniqKey="Goodfellow H">H.R. Goodfellow</name></author><author><name sortKey="Valverde, M A" uniqKey="Valverde M">M.A. Valverde</name></author><author><name sortKey="Gill, D R" uniqKey="Gill D">D.R. Gill</name></author><author><name sortKey="Sepulveda, V" uniqKey="Sepulveda V">V. Sepulveda</name></author><author><name sortKey="Higgins, C F" uniqKey="Higgins C">C.F. Higgins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bond, T D" uniqKey="Bond T">T.D. Bond</name></author><author><name sortKey="Valverde, M A" uniqKey="Valverde M">M.A. Valverde</name></author><author><name sortKey="Higgins, C F" uniqKey="Higgins C">C.F. Higgins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ehring, G R" uniqKey="Ehring G">G.R. Ehring</name></author><author><name sortKey="Osipchuk, Y V" uniqKey="Osipchuk Y">Y.V. Osipchuk</name></author><author><name sortKey="Cahalan, M D" uniqKey="Cahalan M">M.D. Cahalan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miwa, A" uniqKey="Miwa A">A. Miwa</name></author><author><name sortKey="Ueda, K" uniqKey="Ueda K">K. Ueda</name></author><author><name sortKey="Okada, Y" uniqKey="Okada Y">Y. Okada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Greef, C" uniqKey="De Greef C">C. De Greef</name></author><author><name sortKey="Sehrer, J" uniqKey="Sehrer J">J. Sehrer</name></author><author><name sortKey="Viana, F" uniqKey="Viana F">F. Viana</name></author><author><name sortKey="Van Acker, K" uniqKey="Van Acker K">K. van Acker</name></author><author><name sortKey="Eggermont, J" uniqKey="Eggermont J">J. Eggermont</name></author><author><name sortKey="Mertens, L" uniqKey="Mertens L">L. Mertens</name></author><author><name sortKey="Raeymaekers, L" uniqKey="Raeymaekers L">L. Raeymaekers</name></author><author><name sortKey="Droogmans, G" uniqKey="Droogmans G">G. Droogmans</name></author><author><name sortKey="Nilius, B" uniqKey="Nilius B">B. Nilius</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Greef, C" uniqKey="De Greef C">C. De Greef</name></author><author><name sortKey="Van Der Heyden, S" uniqKey="Van Der Heyden S">S. van der Heyden</name></author><author><name sortKey="Viana, F" uniqKey="Viana F">F. Viana</name></author><author><name sortKey="Eggermont, J" uniqKey="Eggermont J">J. Eggermont</name></author><author><name sortKey="De Bruijn, E A" uniqKey="De Bruijn E">E.A. de Bruijn</name></author><author><name sortKey="Raeymaekers, L" uniqKey="Raeymaekers L">L. Raeymaekers</name></author><author><name sortKey="Droogmans, G" uniqKey="Droogmans G">G. Droogmans</name></author><author><name sortKey="Nilius, B" uniqKey="Nilius B">B. Nilius</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marin, M" uniqKey="Marin M">M. Marin</name></author><author><name sortKey="Poret, A" uniqKey="Poret A">A. Poret</name></author><author><name sortKey="Maillet, G" uniqKey="Maillet G">G. Maillet</name></author><author><name sortKey="Leboulenger, F" uniqKey="Leboulenger F">F. Leboulenger</name></author><author><name sortKey="Le Foll, F" uniqKey="Le Foll F">F. le Foll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poulsen, K A" uniqKey="Poulsen K">K.A. Poulsen</name></author><author><name sortKey="Andersen, E C" uniqKey="Andersen E">E.C. Andersen</name></author><author><name sortKey="Hansen, C F" uniqKey="Hansen C">C.F. Hansen</name></author><author><name sortKey="Klausen, T K" uniqKey="Klausen T">T.K. Klausen</name></author><author><name sortKey="Hougaard, C" uniqKey="Hougaard C">C. Hougaard</name></author><author><name sortKey="Lambert, I H" uniqKey="Lambert I">I.H. Lambert</name></author><author><name sortKey="Hoffmann, E K" uniqKey="Hoffmann E">E.K. Hoffmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Torre, A" uniqKey="Torre A">A. Torre</name></author><author><name sortKey="Trischitta, F" uniqKey="Trischitta F">F. Trischitta</name></author><author><name sortKey="Faggio, C" uniqKey="Faggio C">C. Faggio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pasquier, J" uniqKey="Pasquier J">J. Pasquier</name></author><author><name sortKey="Galas, L" uniqKey="Galas L">L. Galas</name></author><author><name sortKey="Boulange Lecomte, C" uniqKey="Boulange Lecomte C">C. Boulange-Lecomte</name></author><author><name sortKey="Rioult, D" uniqKey="Rioult D">D. Rioult</name></author><author><name sortKey="Bultelle, F" uniqKey="Bultelle F">F. Bultelle</name></author><author><name sortKey="Magal, P" uniqKey="Magal P">P. Magal</name></author><author><name sortKey="Webb, G" uniqKey="Webb G">G. Webb</name></author><author><name sortKey="Le Foll, F" uniqKey="Le Foll F">F. le Foll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pasquier, J" uniqKey="Pasquier J">J. Pasquier</name></author><author><name sortKey="Magal, P" uniqKey="Magal P">P. Magal</name></author><author><name sortKey="Boulange Lecomte, C" uniqKey="Boulange Lecomte C">C. Boulange-Lecomte</name></author><author><name sortKey="Webb, G" uniqKey="Webb G">G. Webb</name></author><author><name sortKey="Le Foll, F" uniqKey="Le Foll F">F. Le Foll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pasquier, J" uniqKey="Pasquier J">J. Pasquier</name></author><author><name sortKey="Rioult, D" uniqKey="Rioult D">D. Rioult</name></author><author><name sortKey="Abu Kaoud, N" uniqKey="Abu Kaoud N">N. Abu-Kaoud</name></author><author><name sortKey="Marie, S" uniqKey="Marie S">S. Marie</name></author><author><name sortKey="Rafii, A" uniqKey="Rafii A">A. Rafii</name></author><author><name sortKey="Guerrouahen, B S" uniqKey="Guerrouahen B">B.S. Guerrouahen</name></author><author><name sortKey="Le Foll, F" uniqKey="Le Foll F">F. le Foll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hille, B" uniqKey="Hille B">B. Hille</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gill, D R" uniqKey="Gill D">D.R. Gill</name></author><author><name sortKey="Hyde, S C" uniqKey="Hyde S">S.C. Hyde</name></author><author><name sortKey="Higgins, C F" uniqKey="Higgins C">C.F. Higgins</name></author><author><name sortKey="Valverde, M A" uniqKey="Valverde M">M.A. Valverde</name></author><author><name sortKey="Mintenig, G M" uniqKey="Mintenig G">G.M. Mintenig</name></author><author><name sortKey="Sepulveda, F V" uniqKey="Sepulveda F">F.V. Sepulveda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sardini, A" uniqKey="Sardini A">A. Sardini</name></author><author><name sortKey="Mintenig, G M" uniqKey="Mintenig G">G.M. Mintenig</name></author><author><name sortKey="Valverde, M A" uniqKey="Valverde M">M.A. Valverde</name></author><author><name sortKey="Sepulveda, F V" uniqKey="Sepulveda F">F.V. Sepulveda</name></author><author><name sortKey="Gill, D R" uniqKey="Gill D">D.R. Gill</name></author><author><name sortKey="Hyde, S C" uniqKey="Hyde S">S.C. Hyde</name></author><author><name sortKey="Higgins, C F" uniqKey="Higgins C">C.F. Higgins</name></author><author><name sortKey="Mcnaughton, P A" uniqKey="Mcnaughton P">P.A. McNaughton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weaver, J L" uniqKey="Weaver J">J.L. Weaver</name></author><author><name sortKey="Aszalos, A" uniqKey="Aszalos A">A. Aszalos</name></author><author><name sortKey="Mckinney, L" uniqKey="Mckinney L">L. McKinney</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aleu, J" uniqKey="Aleu J">J. Aleu</name></author><author><name sortKey="Ivorra, I" uniqKey="Ivorra I">I. Ivorra</name></author><author><name sortKey="Lejarreta, M" uniqKey="Lejarreta M">M. Lejarreta</name></author><author><name sortKey="Gonzalez Ros, J M" uniqKey="Gonzalez Ros J">J.M. Gonzalez-Ros</name></author><author><name sortKey="Morales, A" uniqKey="Morales A">A. Morales</name></author><author><name sortKey="Ferragut, J A" uniqKey="Ferragut J">J.A. Ferragut</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, L X" uniqKey="Chen L">L.X. Chen</name></author><author><name sortKey="Wang, L W" uniqKey="Wang L">L.W. Wang</name></author><author><name sortKey="Jacob, T" uniqKey="Jacob T">T. Jacob</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shi, X" uniqKey="Shi X">X. Shi</name></author><author><name sortKey="Wu, S" uniqKey="Wu S">S. Wu</name></author><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y. Yang</name></author><author><name sortKey="Tang, L" uniqKey="Tang L">L. Tang</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Dong, J" uniqKey="Dong J">J. Dong</name></author><author><name sortKey="Lu, B" uniqKey="Lu B">B. Lu</name></author><author><name sortKey="Jiang, G" uniqKey="Jiang G">G. Jiang</name></author><author><name sortKey="Zhao, W" uniqKey="Zhao W">W. Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jablonski, E" uniqKey="Jablonski E">E. Jablonski</name></author><author><name sortKey="Webb, A" uniqKey="Webb A">A. Webb</name></author><author><name sortKey="Hughes, F M" uniqKey="Hughes F">F.M. Hughes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jablonski, E M" uniqKey="Jablonski E">E.M. Jablonski</name></author><author><name sortKey="Mattocks, M A" uniqKey="Mattocks M">M.A. Mattocks</name></author><author><name sortKey="Sokolov, E" uniqKey="Sokolov E">E. Sokolov</name></author><author><name sortKey="Koniaris, L G" uniqKey="Koniaris L">L.G. Koniaris</name></author><author><name sortKey="Hughes, F M" uniqKey="Hughes F">F.M. Hughes</name></author><author><name sortKey="Fausto, N" uniqKey="Fausto N">N. Fausto</name></author><author><name sortKey="Pierce, R H" uniqKey="Pierce R">R.H. Pierce</name></author><author><name sortKey="Mckillop, I H" uniqKey="Mckillop I">I.H. McKillop</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hanahan, D" uniqKey="Hanahan D">D. Hanahan</name></author><author><name sortKey="Weinberg, R A" uniqKey="Weinberg R">R.A. Weinberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Johnstone, R W" uniqKey="Johnstone R">R.W. Johnstone</name></author><author><name sortKey="Ruefli, A A" uniqKey="Ruefli A">A.A. Ruefli</name></author><author><name sortKey="Tainton, K M" uniqKey="Tainton K">K.M. Tainton</name></author><author><name sortKey="Smyth, M J" uniqKey="Smyth M">M.J. Smyth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, X" uniqKey="Yang X">X. Yang</name></author><author><name sortKey="Page, M" uniqKey="Page M">M. Page</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Litman, T" uniqKey="Litman T">T. Litman</name></author><author><name sortKey="Nielsen, D" uniqKey="Nielsen D">D. Nielsen</name></author><author><name sortKey="Skovsgaard, T" uniqKey="Skovsgaard T">T. Skovsgaard</name></author><author><name sortKey="Zeuthen, T" uniqKey="Zeuthen T">T. Zeuthen</name></author><author><name sortKey="Stein, W D" uniqKey="Stein W">W.D. Stein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barakat, S" uniqKey="Barakat S">S. Barakat</name></author><author><name sortKey="Demeule, M" uniqKey="Demeule M">M. Demeule</name></author><author><name sortKey="Pilorget, A" uniqKey="Pilorget A">A. Pilorget</name></author><author><name sortKey="Regina, A" uniqKey="Regina A">A. Regina</name></author><author><name sortKey="Gingras, D" uniqKey="Gingras D">D. Gingras</name></author><author><name sortKey="Baggetto, L G" uniqKey="Baggetto L">L.G. Baggetto</name></author><author><name sortKey="Beliveau, R" uniqKey="Beliveau R">R. Beliveau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barakat, S" uniqKey="Barakat S">S. Barakat</name></author><author><name sortKey="Turcotte, S" uniqKey="Turcotte S">S. Turcotte</name></author><author><name sortKey="Demeule, M" uniqKey="Demeule M">M. Demeule</name></author><author><name sortKey="Lachambre, M P" uniqKey="Lachambre M">M.P. Lachambre</name></author><author><name sortKey="Regina, A" uniqKey="Regina A">A. Regina</name></author><author><name sortKey="Baggetto, L G" uniqKey="Baggetto L">L.G. Baggetto</name></author><author><name sortKey="Beliveau, R" uniqKey="Beliveau R">R. Beliveau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Demeule, M" uniqKey="Demeule M">M. Demeule</name></author><author><name sortKey="Jodoin, J" uniqKey="Jodoin J">J. Jodoin</name></author><author><name sortKey="Gingras, D" uniqKey="Gingras D">D. Gingras</name></author><author><name sortKey="Beliveau, R" uniqKey="Beliveau R">R. Beliveau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pedersen, S F" uniqKey="Pedersen S">S.F. Pedersen</name></author><author><name sortKey="Hoffmann, E K" uniqKey="Hoffmann E">E.K. Hoffmann</name></author><author><name sortKey="Novak, I" uniqKey="Novak I">I. Novak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miyazaki, T" uniqKey="Miyazaki T">T. Miyazaki</name></author><author><name sortKey="Arai, S" uniqKey="Arai S">S. Arai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sveiczer, A" uniqKey="Sveiczer A">A. Sveiczer</name></author><author><name sortKey="Novak, B" uniqKey="Novak B">B. Novak</name></author><author><name sortKey="Mitchison, J M" uniqKey="Mitchison J">J.M. Mitchison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wells, W A" uniqKey="Wells W">W.A. Wells</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cooper, S" uniqKey="Cooper S">S. Cooper</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jorgensen, P" uniqKey="Jorgensen P">P. Jorgensen</name></author><author><name sortKey="Tyers, M" uniqKey="Tyers M">M. Tyers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saucedo, L J" uniqKey="Saucedo L">L.J. Saucedo</name></author><author><name sortKey="Edgar, B A" uniqKey="Edgar B">B.A. Edgar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Conlon, I" uniqKey="Conlon I">I. Conlon</name></author><author><name sortKey="Raff, M" uniqKey="Raff M">M. Raff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Umen, J G" uniqKey="Umen J">J.G. Umen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ernest, N J" uniqKey="Ernest N">N.J. Ernest</name></author><author><name sortKey="Habela, C W" uniqKey="Habela C">C.W. Habela</name></author><author><name sortKey="Sontheimer, H" uniqKey="Sontheimer H">H. Sontheimer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Habela, C W" uniqKey="Habela C">C.W. Habela</name></author><author><name sortKey="Sontheimer, H" uniqKey="Sontheimer H">H. Sontheimer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author><author><name sortKey="Vo, T" uniqKey="Vo T">T. Vo</name></author><author><name sortKey="Hajar, A" uniqKey="Hajar A">A. Hajar</name></author><author><name sortKey="Li, S" uniqKey="Li S">S. Li</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X. Chen</name></author><author><name sortKey="Parissenti, A M" uniqKey="Parissenti A">A.M. Parissenti</name></author><author><name sortKey="Brindley, D N" uniqKey="Brindley D">D.N. Brindley</name></author><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Murray, S" uniqKey="Murray S">S. Murray</name></author><author><name sortKey="Briasoulis, E" uniqKey="Briasoulis E">E. Briasoulis</name></author><author><name sortKey="Linardou, H" uniqKey="Linardou H">H. Linardou</name></author><author><name sortKey="Bafaloukos, D" uniqKey="Bafaloukos D">D. Bafaloukos</name></author><author><name sortKey="Papadimitriou, C" uniqKey="Papadimitriou C">C. Papadimitriou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maulard, C" uniqKey="Maulard C">C. Maulard</name></author><author><name sortKey="Marie, J P" uniqKey="Marie J">J.P. Marie</name></author><author><name sortKey="Delanian, S" uniqKey="Delanian S">S. Delanian</name></author><author><name sortKey="Housset, M" uniqKey="Housset M">M. Housset</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Le Foll, F" uniqKey="Le Foll F">F. Le Foll</name></author><author><name sortKey="Rioult, D" uniqKey="Rioult D">D. Rioult</name></author><author><name sortKey="Boussa, S" uniqKey="Boussa S">S. Boussa</name></author><author><name sortKey="Pasquier, J" uniqKey="Pasquier J">J. Pasquier</name></author><author><name sortKey="Dagher, Z" uniqKey="Dagher Z">Z. Dagher</name></author><author><name sortKey="Leboulenger, F" uniqKey="Leboulenger F">F. Leboulenger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rioult, D" uniqKey="Rioult D">D. Rioult</name></author><author><name sortKey="Pasquier, J" uniqKey="Pasquier J">J. Pasquier</name></author><author><name sortKey="Boulange Lecomte, C" uniqKey="Boulange Lecomte C">C. Boulange-Lecomte</name></author><author><name sortKey="Poret, A" uniqKey="Poret A">A. Poret</name></author><author><name sortKey="Abbas, I" uniqKey="Abbas I">I. Abbas</name></author><author><name sortKey="Marin, M" uniqKey="Marin M">M. Marin</name></author><author><name sortKey="Minier, C" uniqKey="Minier C">C. Minier</name></author><author><name sortKey="Le Foll, F" uniqKey="Le Foll F">F. le Foll</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><noCountry><name sortKey="Abu Kaoud, Nadine" sort="Abu Kaoud, Nadine" uniqKey="Abu Kaoud N" first="Nadine" last="Abu-Kaoud">Nadine Abu-Kaoud</name><name sortKey="Hoarau Vechot, Jessica" sort="Hoarau Vechot, Jessica" uniqKey="Hoarau Vechot J" first="Jessica" last="Hoarau-Véchot">Jessica Hoarau-Véchot</name><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name><name sortKey="Marin, Matthieu" sort="Marin, Matthieu" uniqKey="Marin M" first="Matthieu" last="Marin">Matthieu Marin</name><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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